Journal: Animals : an Open Access Journal from MDPI

Article Title: Multidrug Antimicrobial Resistance and Molecular Detection of mcr-1 Gene in Salmonella Species Isolated from Chicken

doi: 10.3390/ani11010206

Figure Lengend Snippet: Primer sequence for mcr-1 gene detection in Salmonella isolates.

Article Snippet: Ten isolates out of the 82 phenotypically identified Salmonella cultures were randomly selected and were subjected to further testing using Salmonella spp. detection kit (AddBio Inc. Ltd., Daejeon, South Korea) using PCR molecular methods for the detection of the invA gene.

Techniques: Sequencing

Journal: Animals : an Open Access Journal from MDPI

Article Title: Multidrug Antimicrobial Resistance and Molecular Detection of mcr-1 Gene in Salmonella Species Isolated from Chicken

doi: 10.3390/ani11010206

Figure Lengend Snippet: Antimicrobial susceptibility profile of Salmonella isolates ( n = 82) in the present study.

Article Snippet: Ten isolates out of the 82 phenotypically identified Salmonella cultures were randomly selected and were subjected to further testing using Salmonella spp. detection kit (AddBio Inc. Ltd., Daejeon, South Korea) using PCR molecular methods for the detection of the invA gene.

Techniques:

Journal: Animals : an Open Access Journal from MDPI

Article Title: Multidrug Antimicrobial Resistance and Molecular Detection of mcr-1 Gene in Salmonella Species Isolated from Chicken

doi: 10.3390/ani11010206

Figure Lengend Snippet: Multidrug resistance patterns among mcr-1 positive Salmonella spp. isolates in the present study.

Article Snippet: Ten isolates out of the 82 phenotypically identified Salmonella cultures were randomly selected and were subjected to further testing using Salmonella spp. detection kit (AddBio Inc. Ltd., Daejeon, South Korea) using PCR molecular methods for the detection of the invA gene.

Techniques:

Journal: Animals : an Open Access Journal from MDPI

Article Title: Multidrug Antimicrobial Resistance and Molecular Detection of mcr-1 Gene in Salmonella Species Isolated from Chicken

doi: 10.3390/ani11010206

Figure Lengend Snippet: PCR amplification of antimicrobial resistance mcr-1 gene of Salmonella isolates. In Salmonella (S6 to S10) isolates, fragments of ( A ) 1197 bp and ( B ) 799 bp were detected. Lane M: DNA ladder. Lane NC: negative control. Lanes S1 to S5 represents mcr-1 negative isolates while lanes S6 to S10 represents mcr-1 positive Salmonella isolates SAUVM S6 to SAUVM S10.

Article Snippet: Ten isolates out of the 82 phenotypically identified Salmonella cultures were randomly selected and were subjected to further testing using Salmonella spp. detection kit (AddBio Inc. Ltd., Daejeon, South Korea) using PCR molecular methods for the detection of the invA gene.

Techniques: Amplification, Negative Control

Journal: Animals : an Open Access Journal from MDPI

Article Title: Multidrug Antimicrobial Resistance and Molecular Detection of mcr-1 Gene in Salmonella Species Isolated from Chicken

doi: 10.3390/ani11010206

Figure Lengend Snippet: Phylogeny analysis showing ancestral origin and diversification of MCR-1 and MCR-1 like proteins. The BLAST search tool ( https://blast.ncbi.nlm.nih.gov/Blast.cgi ) was used to retrieve the homologous sequences of the MCR-1 and MCR-1 like proteins from the NCBI database using amino acid sequences of five SAUVM MCR-1 proteins. Sequences were categorized into MCR-1 and MCR-1 like proteins of Salmonella, E. coli , strains containing LptA (formerly named EptA) and others. The maximum likelihood method of MEGA X was used to construct a phylogenetic tree using aligned sequences of MCR-1 from CLUSTALW.

Article Snippet: Ten isolates out of the 82 phenotypically identified Salmonella cultures were randomly selected and were subjected to further testing using Salmonella spp. detection kit (AddBio Inc. Ltd., Daejeon, South Korea) using PCR molecular methods for the detection of the invA gene.

Techniques: Construct

Journal: Animals : an Open Access Journal from MDPI

Article Title: Multidrug Antimicrobial Resistance and Molecular Detection of mcr-1 Gene in Salmonella Species Isolated from Chicken

doi: 10.3390/ani11010206

Figure Lengend Snippet: Transmembrane topology prediction of Salmonella SAUVM S6 MCR-1 protein. TMHMM server v.2.0 ( http://www.cbs.dtu.dk/services/TMHMM/ ) was used to predict the ( A ) transmembrane helices of MCR-1 proteins and the image showed TMHMM posterior probabilities of SAUVM S6. ( B ) The topology was shown as the position of the transmembrane helices differentiated by “i” and “o” when the loop is on the inside and outside, respectively.

Article Snippet: Ten isolates out of the 82 phenotypically identified Salmonella cultures were randomly selected and were subjected to further testing using Salmonella spp. detection kit (AddBio Inc. Ltd., Daejeon, South Korea) using PCR molecular methods for the detection of the invA gene.

Techniques:

Journal: Animals : an Open Access Journal from MDPI

Article Title: Multidrug Antimicrobial Resistance and Molecular Detection of mcr-1 Gene in Salmonella Species Isolated from Chicken

doi: 10.3390/ani11010206

Figure Lengend Snippet: Modelled structures of Salmonella SAUVM MCR-1 proteins and validation. ( A ) Three dimensional (3D) modeling of Salmonella SAUVM MCR-1 proteins (SAUVM S6 to SAUVM S10) were performed using I-TASSER, which functions by identifying structure templates from the PDB library. The confidence of each model was quantitatively measured by C-score. From these models of MCR-1 proteins, the Salmonella SAUVM S10 model was randomly selected, ( B ) analyzed and structurally validated with the Ramachandran plot assessment server (RAMPAGE).

Article Snippet: Ten isolates out of the 82 phenotypically identified Salmonella cultures were randomly selected and were subjected to further testing using Salmonella spp. detection kit (AddBio Inc. Ltd., Daejeon, South Korea) using PCR molecular methods for the detection of the invA gene.

Techniques: Biomarker Discovery